Sustracted library obtained from mutant sugarcane variety B 4362 resistant to rust

Sustracted library obtained from mutant sugarcane variety B 4362 resistant to rust

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The hypersensitive response is one of the most powerful mechanisms for which the plants resist pathogen attack.Mutations carried out previously on the variety B4362, of sugarcane, originated five mutants that express this mechanism towards aboriginal flag beanie the attack of rust (Puccinia melanocephala Syd.).

By means of a subtractive hybridization among the cDNA obtained starting from the resistant clone inoculated with rust and a pool of cDNA of the susceptible variety (B4362) inoculated and of the resistant clone not inoculated, it was possible to reduce the number of genes expressed during the infection with the fungus.A subtractive library was carried out where we hope rms beauty luminizer x quad that most of the genes are involved in the hypersensitive response that present these mutants towards the infection of the pathogen.Key words: Subtractive hybridization, hypersensitive response, Puccinia melanocephala Syd.